Polyhydroxyalkanoates (PHAs) are produced by a wide variety of bacteria as an intracellular storage material of carbon and energy. PHA synthase (PhaC) is the key enzyme in the production of PHAs.Basically, my project involve this enzyme from Pseudomonas sp. USM4-55. It is a Gram negative bacterium that is capable of accumulating short and medium chain length polymer. phaC1 and phaC2 genes of Pseudomonas sp. USM4-55 have already been cloned.The phaC1 gene was subcloned again to optimize soluble protein production in Escherichia coli. Proteins was then purified with the help of affinity tags.Currently I'm optimizing the process to obtain sufficient sample to be screened for crystallization conditions with the goal of single crystal X-ray diffraction.
* Aim : A single crystal for structure prediction..InsyaALLAH~
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